Bio-Rad Bio-Plex Pro™ Human Chemokine Assays - Manual (Page 24)

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Table 12. Read the plate using the appropriate instrument settings.
9. Read Plate
Bio-Plex Manager ™ software is recommended for all Bio-Plex Pro ™ assay
data acquisition and analysis. Instructions for Luminex xPoNENT software
are also included. For instructions using other xMAP system software
packages, contact Bio-Rad Technical Support or your regional Bio-Rad
field applications specialist.
Prepare Protocol in Bio-Plex Manager Software
Version 6.0 and Higher
The protocol should be prepared in advance so that the plate is read as
soon as the experiment is complete.
A protocol file specifies the analytes in the assay, the plate wells to be
read, sample information, the values of standards and controls, and
instrument settings.
Bio-Plex Manager software versions 6.0 and higher contain protocols for
most Bio-Plex
® assays. Choose from available protocols or create a new
protocol. To create a new protocol, select File, then New from the main
menu. Locate and follow the steps under Protocol Settings.
1. Click Describe Protocol and enter information about the assay
(optional).
2. Click Select Analytes and create a new panel. Visually confirm the
selected analytes and proceed to step 3.
Instrument RP1 (PMT) DD Gates Bead Events
Bio-Plex 100, 200* Low 5,000 (low), 25,000 (high) 50
Bio-Plex 3D* Standard Select MagPlex beads 50
Bio-Plex ® MAGPIX ™ N/A, use default instrument settings
* or similar Luminex-based system.