Bio-Rad Bio-Plex Pro™ Human Chemokine Assays - Manual (Page 12)

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2. Select OK and follow the software prompts for step-by-step
instructions for CAL1 and CAL2 calibration.
Note: In Bio-Plex Manager version 6.1 and higher, startup, warm up,
and calibration can be performed together by selecting the Start up and
calibrate icon.
3. Prepare Wash Method
Bio-Plex Pro ™ assays are compatible with both magnetic separation and
vacuum filtration methods. However, for best results, we recommend
performing the assays in a flat bottom plate with magnetic separation.
Setting up the Bio-Plex Pro or Bio-Plex Pro II
Wash Station
The wash station should be primed before use. For more information, refer
to the Bio-Plex Pro Wash Stations Quick Guide (bulletin #5826).
1. Install the appropriate plate carrier on the wash station.
2. Use the Prime procedure to prime channel 1 with 1x wash buffer.
Setting up the Bio-Plex Handheld Magnetic Washer
Place an empty flat bottom plate on the magnetic washer by sliding
it under the retaining clips. Push the clips inward to secure the plate.
Make sure the plate is held securely. If needed, the clips can be adjusted
for height and tension. For detailed instructions, refer to the user guide
(bulletin #10023087).
Table 3. Summary of compatible wash stations and plate types.
Wash Method
Wash Station Assay Plate
Magnetic separation Bio-Plex Pro Flat bottom plate
Bio-Plex Pro II (use MAG programs)
Bio-Plex ® handheld magnetic washer
Vacuum filtration Bio-Plex Pro II (use VAC programs) Filter plate
Vacuum manifold (manual)