Appendix: non-human primate (nhp) cross-reactivity – Bio-Rad Bio-Plex Pro™ Human Chemokine Assays User Manual
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Appendix: Non-Human Primate (NHP)
Cross-Reactivity
The human chemokine assays were found to be cross-reactive with
two common NHP species, cynomolgus (Cyno) macaque and rhesus
macaque. other NHP species were not tested. The degree of cross-
reactivity was determined based on the ability of each assay to detect
native analyte in serum, plasma and/or culture media from mitogen
stimulated peripheral blood mononuclear cells (PBMC).
Assay signal (MFI) for the most highly cross-reactive samples were compared
against the human standard curve and scored as shown in the Table 14.
Table 14. NHP cross-reactivity of the human chemokine assays.
Analyte
Cyno
Rhesus
Analyte
Cyno
Rhesus
6Ckine/CCL21
–
–
IL-8
+++
+++
BCA-1/CXCL13
+++
+++
IP-10/CXCL10
+++
+++
CTACK/CCL27
–
– I-TAC/CXCL11
+++
+++
ENA-78/CXCL5
+
+
MCP-1/CCL2
+++
+++
Eotaxin/CCL11
+
+
MCP-2/CCL8
++
++
Eotaxin-2/CCL24
–
–
MCP-3/CCL7
–
–
Eotaxin-3/CCL-26
–
–
MCP-4/CCL13
+++
+++
Fractalkine/CX3CL1 +++
+ MDC/CCL22
+
–
GCP-2/CXCL6
–
–
MIF
+++
+++
GM-CSF
+++
+++
MIG/CXCL9
–
–
Gro-a/CXCL1
–
– MIP-1a/CCL3
+++
+++
Gro-b/CXCL2
+
+
MIP-1δ/CCL15
–
+
I-309/CCL1
–
– MIP-3a/CCL20
–
–
IFN-g
–
– MIP-3b/CCL19
++
++
IL-10
+++
+++ MPIF-1/CCL23
–
–
IL-16
+
+ SCYB16/CXCL16
–
+
IL-1b
+++
+++ SDF-1a+b/CXCL12 +
+
IL-2
–
– TARC/CCL17
–
–
IL-4
++
+
TECK/CCL25
+
+
IL-6
+++
+++ TNF-a
+
–
–
No NHP cross-reactivity observed. Assay signal below S6 on the standard curve.
+ Some NHP cross-reactivity observed. Assay signal above S6.
++ Moderate NHP cross-reactivity observed. Assay signal above S5.
+++ Strong NHP cross-reactivity observed. Assay signal above S3.