Bio-Rad Microplate Manager Software User Manual

Page 30

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Setting Up a Protocol

22

Section, the Control Validation Section, the Cutoff Values Section, and the Matrix
Data including the Sample info.

In the Information section, the report template, technician, plate, reader, and reading
parameters are given.

The custom report template is uniquely identified with a name, part number and
revision number. Each custom report template is released as a controlled document
with its own part number and revision level. All validation procedures and results are
included with each release.

In the Control Validation Section, the results for the positive and negative controls
are given, as specified in the Analysis Settings Worksheet. The number of valid
controls and the mean absorbance value for each control group (positive and
negative) are shown. These values are highlighted in red, if the control group has
failed validation.

The Calculated Cutoff Values for identifying the positive, negative and indeterminate
samples are shown in the third section. These are calculated as specified in the
Analysis Settings Worksheet.

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