Setting up a protocol, Analyzing data using a standard curve, Analyzing data using cutoffs – Bio-Rad Microplate Manager Software User Manual

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Microplate Manager 6

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Exporting Data ........................................................................................ 18

Exporting Reports ................................................................................... 19

Exporting Custom Screening Reports (BSE, TSE, TSE, NSP) .............. 20

Procedure for Custom Export Reports.................................................... 20

BSE Custom Screening Report .............................................................. 21

Setting up a Protocol ................................................... 24

Setting Up A Template ........................................................................25

Entering Equations.................................................................................. 26

Entering and Formatting Experimental Info ............................................ 27

Select and Customize Curve Fit Plot ...................................................... 28

Setting up the Report Contents............................................................... 30

Saving the Protocol .............................................................................31

Analyzing Data Using a Standard Curve.................... 32

Standard Curve Analysis Options........................................................33

Linear Fit ................................................................................................. 33

Zero-intercept Linear............................................................................... 34

Semi-log Fit ............................................................................................. 35

Log/log Fit ............................................................................................... 35

Log/log Fit ............................................................................................... 36

Quadratic.................................................................................................36

Log/Logit and 4-Parameter Fit ................................................................ 37

Point to Point........................................................................................... 39

Cubic Spline ............................................................................................ 39

Interpolation Using a Previous Standard Curve ..................................40

Customizing Standard Curve Plots......................................................41

Equations and Calculations .................................................................42

Analyzing Data Using Cutoffs..................................... 45

Using Equations for Setting Cutoffs.....................................................46

Cutoff Examples for Assays with Controls...........................................47

Setting Up a Percent Control Cutoff Analysis Report .........................48

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