Point to point, Cubic spline – Bio-Rad Microplate Manager Software User Manual
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Analyzing Data Using a Standard Curve
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Point to Point
In the Point to Point method of analysis, the data points are simply connected. No
fitting of the data points to a line or curve is done.
For reactions where there is a lag time or a plateau, it is often convenient to look at
the data using this plot in order to see the true linear range of the assay.
Cubic Spline
Cubic spline is a piecewise polynomial approximation in which a set of data points
are joined by a series of curve fits. These multiple curve fits are smoothed by using
a cubic fit
y = Ax + Bx2 + Cx3 + D
This type of curve fit is less affected when any one of the standards has a poor value.
Because multiple curve fits are performed with this function, the fit parameters are
not shown with the graph.