Hudson Robotics Micro10x - Manual (Page 28)

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Section 4 – Micro10x Validation
Section 4.1 – General Validation After Cleaning
To Ensure that the unit is dispensing properly after cleaning you will need to perform a
validation dispense. To perform a validation dispense you will need the following items:

Clear 96-well Polypropylene Plate

Bulk reagent to dispense (preferably not clear)

Optional- Sartorius WZA225-CW balance or similar with at least a 0.001mg
readability.
Setup a dispense program in the Micro10x to dispense 100ul into the 96-well plate.
Make sure to prime the system properly so the unit is dispensing the bulk reagent without
any air bubbles. Dispense into the entire plate, once the unit is finished dispensing you
will inspect the plate. The inspect the plate you will raise the plate to eye level and
visually inspect each well with the next in row A and H of the Plate. You want to make
sure that the fluid makes a level horizontal line across the entire plate. If the fluid levels
are not even, remove the manifold and clean/sonicate to clean. Run the test again after
the manifold is re-cleaned.
Optional:
Before dispensing into the validation plate you will put it on the balance and tare the
weight of the empty plate. Now take the plate and place it on the Micor10X nest and run
the validation program. Once the program is completed you will weigh the plate on the
balance. The Plate should weigh the following:
1uL of water ~ 1mg
A 100ul/well 96-well plate ~ 9600mg
~2% tolerance @ 100ul
Plate should weigh 9800mg - 9400mg