ANKOM XT15 Series - Manual (Page 10)

Operator’s Manual
pg. 10
Rev F 12/08/14
Fat Extraction Calculations
Crude Fat Calculation
Crude Fat contained within a food or feed sample can be calculated using the following formula:
% Crude Fat = 100 x (W
2
– W
3
)
W
1
Where: W
1
= Original weight of sample
W
2
= Weight of pre-extraction dried sample and filter bag
W
3
= Weight of dried sample and filter bag after extraction
For Liquid Samples, W
2
and W
3
in the equation above will include the weight of Diatomaceous Earth (DE).
For Meat Samples with > 15% fat and plant samples with > 20% fat:
% Crude Fat = 100 x ((W
2
– W
4
) – W
3
)
W
1
Where: W
4
= Weight of the weigh tin
Total Fat Calculation
If hydrolysis is done prior to fat extraction, then Total Fat contained within a food or feed sample can be calculated
using the following formula:
% Total Fat = 100 x (W
2
– (W
3
+ (C
1
– C
2
)))
W
1
Where: W
1
= Original weight of sample
W
2
= Weight of dried sample, filter bag, and Diatomaceous Earth (DE) after hydrolysis
W
3
= Weight of dry extracted sample, filter bag, and DE
C
1
= Blank filter bag dry weight after hydrolysis
C
2
= Blank filter bag weight after extraction
The Hydrolysis procedure can be found in the ANKOM HCl Operator's Manual.
Fat Extraction Support Items
The following support items are needed to perform the fat extraction procedure:
Item Recommended Product
Electronic Balance with four-place readout ANKOM #TB Balance Hardware
ANKOM #TBS Balance Software
Filter Bags ANKOM #XT4
Bag Holder (used for adding sample to an empty filter bag) ANKOM #101.2
Heat Sealer for sealing the filter bags ANKOM #1915 (120V), #1920 (220V)
Solvent Resistant Marker ANKOM #F08
Desiccant Pouch or Weigh Tin Desiccator for drying ANKOM #X45, #X49
Dryer (capable of temperatures from 60-120°C) - optional ANKOM #RD (120V), #RDI (220V)
Oven for drying (capable of maintaining 102°C±2°)
Ventilated oven trays to allow for sufficient oven air flow
Weigh Tins (used with samples of > 20% fat)
Sample
Spoon