Operator’s manual – ANKOM XT15 Series User Manual
Page 14
Operator’s Manual
pg. 14
Rev F 12/08/14
Sample Preparation Procedure for Crude Fat –
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To prepare liquid samples for fat extraction, follow the procedure detailed below.
1. Using a Solvent Resistant Marker, number all of the empty filter bags you will
use during the fat extraction procedure. Although it is not necessary in the
determination of fat, it is recommended that you weigh and record the weights of
all the filter bags prior to filling them in order to calculate the moisture value for
the samples.
% Moisture = 100 x ((Filter Bag Weight + Sample Weight) - Weight after drying)
Sample Weight
2. Place an empty filter bag in the Bag Holder in an open position.
3. Tare the weight of the empty filter bag and the holder together.
4. Add Diatomaceous Earth (DE).
4.1
Add 1.0 – 1.25 g of DE to the filter bag.
4.2
Using a pencil eraser (or similar sized utensil) make an indentation or a
space in the DE into which the liquid sample can be added using a pipette.
4.3
Record the weight and tare.
5. Pipette 1.5 – 2 g of liquid sample into the DE indentation in the filter bag.
(Do NOT use more liquid sample than can be absorbed by the DE.)
6. Record the weight of the sample (W
1
).
7. Set the Heat Sealer dial to 6. (The setting may vary from sealer to sealer.)
8. Seal the filter bag within 4mm of its open end. Keep the
sealer arm down for 2 – 3 seconds after the red sealer light
turns off (to cool the seal). The seal can be seen as a solid
melted stripe along the top edge of the filter bag (as shown
to the right). If the seal is not strong, re-seal the bag.
9. Repeat steps 1 – 8 for all filter bags that will be used in the ANKOM
XT15
Extraction System. (Up to 15
bags can be processed during one procedure.)
Seal