Luminex 100 IS User Manual, Version 2.1 - Manual (Page 153)

x MAP Technology Troubleshooting
PN 89-00002-00-061 Rev. A 9 - 7
Insufficient Bead Count
• Do not select Cancel during sample acquisition. Selecting the
Cancel function while a sample is acquiring causes an error
message to occur.
• Ensure that you selected the correct template.
• If applicable, see if all of the tests (beads) were added to the
samples.
• See if the samples were properly mixed before they were
dispensed into the wells.
• See if you loaded the sample to the correct well on the plate.
• Check if the sample volume added to the well is correct.
• Ensure that the samples were completely resuspended prior to
running on the analyzer.
• Remove and sonicate or flush the XYP instrument Sample Probe
to remove possible clogs.
• Check if the XYP instrument Sample Probe is properly aligned.
• Complete the following analyzer functions from the Maintenance
tab: Drain, Backflush, and Alcohol Flush.
• Run the system control microspheres.
• Check to see if the delta cal temperature < 3.0 degrees.
Recalibrate if greater and retry.
• If the system controls pass, contact the kit manufacturer.
Temperature Divergence
from Calibration
Temperature
• Calibrate and verify the system.
• If this message still appears following calibration, contact
Luminex Customer Support.
We recommend that you install the system in a temperature-
controlled environment.
Failed Control in Batch
This message occurs when one or more of the assay controls are not
within the expected range.
• Ensure that you selected the correct template.
• Ensure that the correct control lot number and target values were
selected and entered correctly.
• Verify that the system is functioning properly by running the
system controls.
• If the system controls pass, contact the kit manufacturer.
Failed Curve Fit
This message occurs when the calculation could not occur for the
assay standards for data interpretation.
• Ensure that you selected the correct template.