Leica Biosystems Premium blade holder - Manual (Page 9)

9 Leica Premium blade holder
1. Sectioning – Premium blade holder
Clearance angle adjustment
1. The clearance angle scale is located on the
left side of the blade holder.
2. Release the blade holder by turning the No.
4 Allen screw (23) counterclockwise. Select
a clearance angle of 0°. To do so, align the
number 0 to the index mark (25) and tighten
the Allen screw (23). If the sectioning results
are not satisfactory, increase the clearance
angle in 1° increments until you achieve op-
timum results.
25
23
• A clearance angle that is too steep or too shallow may lead to poor sectioning results
and can possibly damage the specimen.
Fig. 11
The position of the knife edge relative to the specimen will change when the clearance angle is
adjusted. For this reason, always place the specimen above the knife when adjusting the clear‑
ance angle. Otherwise, the specimen could hit the knife while being raised. Carefully check the
distance between the block face and the cutting surface before taking the first section.
Settings of 2° ‑ 5° (premium blade
holder) are well suited for most appli‑
cations.