Leica Biosystems ASP300 - Manual (Page 58)

58 Instruction manual V 1.3 – 12/2003
6.4 Typical fill or drain problems
Fill and drain problems may be due to several causes:
1. There is insufficient reagent
• Verify that both the reagent bottles and the wax stations have
sufficient reagent.
2. Insufficient pressure or vacuum
The Leica ASP300 is unable to create sufficient vacuum (for a fill) or
pressure (for a drain) in the retort.
• Verify that the reagent bottle in question is correctly inserted in
the reagent cabinet.
• Verify that all reagent bottles are fully pushed home into their
sockets.
• Check whether there is some debris under the retort lid seal
causing a leak.
3. There is a blockage in the air or reagent lines
If there is reagent in the retort that cannot be drained, try to remove
the blockage as follows:
• Heat the retort to the maximum possible temperature (consistent
with the reagent currently in the retort, if any) and leave it at this
temperature for at least 15 minutes.
• Whilst the retort is at maximum temperature, attempt to fill or
drain.
• If the retort is empty:
Run the extended retort clean program. Use the maximum set-
tings for number of clean cycles and temperature (consistent
with the type of reagents/solvents used in the clean program).
If you cannot eliminate the problem by carrying out the above
listed steps, call Leica Technical Service
6. Trouble shooting