Waters ACQUITY UPLC CSH130 C18 1.7 μm and XP 2.5 μm Columns - Manual

[ CARE AND USE MANUAL ]
ACQUITY UPLC CSH130 C
18
Columns 1
Thank you for choosing a Waters ACQUITY UPLC ® CSH ™ column.
ACQUITY UPLC CSH130 C
18
, 1.7 µm and XP 2.5 µm columns feature
Waters Charged Surface Hybrid (CSH) Technology which provides
excellent peak shape, high efficiency and loading capacity for basic
compounds when using acidic, low ionic strength mobile phases.
This same particle technology is used in the XSelect
™ CSH family of
HPLC columns, thus enabling seamless transferability between HPLC
and UPLC
® system platforms. The ACQUITY UPLC CSH130 packing
materials were designed specifically for use with the ACQUITY UPLC
systems and are manufactured in a cGMP, ISO 9001 certified
manufacturing facility using ultra pure reagents. Each batch of
ACQUITY UPLC CSH130 C
18
material is tested chromatographically
with acidic, basic, and neutral analytes as part of qualification for
use in peptide mapping. The results are held to narrow specification
ranges to assure excellent, reproducible performance. ACQUITY UPLC
CSH130 C
18
batches are also QC tested with a gradient separation
of a tryptic digest of cytochrome c using 0.1% formic acid containing
eluents. Finally, every shipped column is individually tested for packed
bed efficiency and a Performance Chromatogram and Certificate of
Batch Analysis are provided on the eCord
™ intelligent chip. ACQUITY
UPLC CSH130 C
18
columns were designed and tested specifically for
use on ACQUITY UPLC systems. ACQUITY UPLC CSH130 C
18
columns
will exhibit maximum chromatographic performance and benefits ONLY
when used on holistically-designed ACQUITY UPLC systems since these
systems and columns were created and designed to operate together.
For these reasons, Waters cannot support the use of ACQUITY UPLC
columns on any system other than an ACQUITY UPLC system.
CONTENTS
I. GETTING STARTED
a. Column Connectors
b. Column Installation
c. Column Equilibration
d. eCord Installation
e. Initial Column Efficiency Determination
f. VanGuard Pre-Columns
II. COLUMN USE
a. Sample Preparation
b. pH Range
c. Solvents
d. Pressure
e. Temperature
III. COLUMN CLEANING, REGENERATING AND STORAGE
a. Cleaning and Regeneration
b. Storage
IV. INTRODUCING ECORD INTELLIGENT CHIP TECHNOLOGY
V. REPRESENTATIVE TEST CHROMATOGRAPH AND CONDITIONS
FOR SEPARATION OF PROTEIN DIGEST
VI. ADDITIONAL INFORMATION
ACQUITY UPLC CSH130 C
18
1.7 µm AND XP 2.5 µm COLUMNS