Waters XSelect HSS XP 2.5 µm Columns - Manual

XSelect HSS XP 2.5 µm Columns 1
[ CARE AND USE MANUAL ]
CONTENTS
I. INTRODUCTION
II. GETTING STARTED
a. Column Connection
b. Column Installation
c. Minimizing Band Spread Volume
d. Measuring Band Spread Volume
e. Measuring System Dwell Volume
f. Column Equilibration
g. Solvent Conversion for Normal-Phase Use
h. eCord Installation
i. Initial Column Efficiency Determination
j. VanGuard Pre-Columns
III. COLUMN USE
a. Sample Preparation
b. pH Range
c. Solvents
d. Pressure
e. Temperature
IV. COLUMN CLEANING, REGENERATION AND STORAGE
a. Cleaning and Regeneration
b. Storage after Reversed-Phase Use
c. Storage after HILIC Use
V. eCORD INTELLIGENT CHIP TECHNOLOGY
a. Introduction
b. Installation
c. Manufacturing
d. Column Use
VI. ADDITIONAL INFORMATION
a. Tips for Maximizing XSelect HSS XP 2.5 µm Column Lifetime
b. Troubleshooting Questions
c. Recommended Flow Rates and Anticipated Backpressures for
Reversed-Phase XSelect HSS XP 2.5 µm Columns
XSELECT HSS XP 2.5 µm COLUMNS
I. INT RODUCTION
Thank you for choosing a Waters XSelect ™ High Strength Silica [HSS]
eXtended Performance [XP] 2.5 µm Column. The manufacture of
XSelect HSS XP 2.5 µm Columns begins with ultrapure reagents and
are manufactured in a cGMP, ISO 9001 certified facility to control the
chemical composition and purity of the final product. Well-controlled
manufacturing processes result in industry-leading batch-to-batch
reproducibility. Every column is individually tested. A Performance
Chromatogram and Certificate of Batch Analysis are provided on the
eCord
™ Intelligent Chip.
XSelect HSS XP 2.5 µm Columns are based on the same base
particle technology and bonded phase chemistry as 1.8 µm ACQUITY
UPLC
® HSS Columns as well as XSelect HSS 3.5 and 5 µm HPLC
Columns, thus enabling seamless transferability between HPLC,
UHPLC and UPLC
® platforms.
XSelect HSS XP 2.5 µm Columns will exhibit maximum
chromatographic performance when used on a member of the
ACQUITY UPLC System family.