Waters XBridge XP 2.5 µm Columns - Manual

XBridge XP 2.5 µm Columns 1
[ CARE AND USE MANUAL ]
CONTENTS
I. INTRODUCTION
II. GETTING STARTED
a. Column Connection
b. Column Installation
c. Minimizing Band Spread Volume
d. Measuring Band Spread Volume
e. Measuring System Dwell Volume
f. Column Equilibration
g. eCord Installation
h. Initial Column Efficiency Determination
i. VanGuard Pre-Columns
III. COLUMN USE
a. Sample Preparation
b. pH Range
c. Solvents
d. Pressure
e. Temperature
IV. COLUMN CLEANING, REGENERATION AND STORAGE
a. Cleaning and Regeneration
b. Storage after Reversed-Phase Use
c. Storage after HILIC Use
V. eCORD INTELLIGENT CHIP TECHNOLOGY
a. Introduction
b. Installation
c. Manufacturing
d. Column Use
VI. ADDITIONAL INFORMATION
a. Tips for Maximizing XBridge XP 2.5 µm Column Lifetime
b. Troubleshooting Questions
c. Recommended Flow Rates and Anticipated Backpressures for
Reversed-Phase XBridge XP 2.5 µm Columns
d. Getting Started with XBridge HILIC XP 2.5 µm Columns
e. Getting Started with XBridge Amide XP 2.5 µm Columns
XBRIDGE XP 2.5 µm COLUMNS
I. INT RODUCTION
Thank you for choosing a Waters XBridge ® Ethylene Bridged Hybrid
[BEH] eXtended Performance [XP] 2.5 µm Column. The manufacture
of XBridge XP 2.5 µm Columns begins with ultrapure reagents and
are manufactured in a cGMP, ISO 9001 certified facility to
control the chemical composition and purity of the final product.
Well-controlled manufacturing processes result in industry-leading
batch-to-batch reproducibility. Every column is individually tested.
A Performance Chromatogram and Certificate of Batch Analysis are
provided on the eCord
™ Intelligent Chip.
XP 2.5 µm Columns are based on the same base particle technology
and bonded-phase chemistry as 1.7 µm ACQUITY UPLC
® Columns as
well as XBridge 3.5, 5 and 10 µm HPLC Columns, thus enabling
seamless transferability between HPLC, UHPLC and UPLC
® platforms.
XBridge XP 2.5 µmColumns will exhibit maximum chromatographic
performance when used on a member of the ACQUITY UPLC
System family.