Waters Protein Separation Technology ACQUITY UPLC BEH300, C4, 1.7 µm Columns - Manual (Page 9)

[ Care and Use ManUal ]
Protein Separation Technology ACQUITY UPLC BEH300, C
4
, 1.7 �m
9
b. Storage
For periods longer than four days at room temperature, store the
column in 100% acetonitrile. Immediately after use with elevated
temperatures and/or at pH extremes, store in 100% acetonitrile for
the best column lifetime. Do not store columns in highly aqueous
(<20% organic) mobile phases, as this may promote bacterial growth.
If the mobile phase contained a buffer salt, flush the column with
10 column volumes of HPLC grade water (see Table 1 for common
column volumes) and replace with 100% acetonitrile for storage.
Failure to perform this intermediate step could result in precipitation
of the buffer salt in the column or system when 100% acetonitrile is
introduced. Completely seal column to avoid evaporation and drying
out of the bed.
Note: If a column has been run with a mobile phase that contains
formate (e.g., ammonium formate, formic acid, etc.) and is then
flushed with 100% acetonitrile, slightly longer equilibration times
may be necessary when the column is re-installed and run again with
a formate-containing mobile phase.
VII. IntroduCInG eCord InteLLIGent CHIP teCHnoLoGY
a. Introduction
The eCord intelligent chip is a new technology that will provide
the history of a column’s performance throughout its lifetime. The
eCord will be permanently attached to the column to assure that the
column’s performance history is maintained in the event that the
column is moved from one instrument to another.
Figure 3: Waters eCord Intelligent Chip
At the time of manufacture, tracking and quality control information
will be downloaded to the eCord. Storing this information on the chip
will eliminate the need for a paper Certificate of Analysis. Once the
user installs the column, the software will automatically download
key parameters into a column history file stored on the chip. In
this manual, we explain how the eCord will provide a solution for
easily tracking the history of the columns, reduce the frustration of
paperwork trails, and give customers the reassurance that a well
performing column is installed onto their instruments.
Figure 4: eCord Inserted into Side of Column Heater
b. Installation
Install the column into the column heater. Plug the eCord into the
side of the column heater. Once the eCord is inserted into the column
heater the identification and overall column usage information will
be available in ACQUITY Console, allowing the user to access column
information on their desktop.
c. Manufacturing Information