Waters Symmetry Columns - Manual (Page 7)

[ Care and Use ManUal ]
Symmetry Columns 7
c. Cartridge Installation
Refer to the installation illustration in Figure 7. Unscrew the end connectors from the old cartridge column, leaving them connected to the inlet
and outlet lines of the instrument.
Figure 7: Installing a Symmetry Cartridge Column with a Sentry Guard Column
Attach the new cartridge column between the connectors so that the direction of the flow arrow on the label is pointing towards the detector.
Finger-tighten all fittings.
b. Band Spreading Minimization
Figure 6 shows the influence of tubing internal diameter on system band spreading and peak shape. As can be seen, the larger tubing diameter
causes excessive peak broadening and lower sensitivity.
Figure 6: Effect of Connecting Tubing on System
Diluted/Distorted Sample Band
0.005 inches
0.020 inches
0.040 inches
0.25 mm (0.009 inch) Tubing
Spacer C-Clip
Symmetry™ Cartridge Column
Sentry™ Guard Column
End Connector