Care and use manual – Waters Protein-Pak HR Ion-Exchange Glass Columns User Manual
Page 8
[ Care and Use ManUal ]
Protein-Pak HR Ion-Exchanged Glass Columns
8
Column: Protein-Pak DEAE 8HR AP
1. Adenosine
2. Carbnic Anhydrase
3. Human Transferrin
4. Ovalbumin
5. Soybean Trypsin Inhibitor
min
Column: Protein-Pak SP 8HR AP-1
1. Myogiobin
2. Ribonuciease A
3. Chymotrypsinogen A
4. Cytochrome C
5. Lysozyme
min
Table 5: Protein Mix for Functional Test Conditions
Figure 2: Typical Chromatograms Using Functional Test Conditions
Mix
Amount of Protein
Solution in Mix
Sigma Catalog
Number
Column: Protein-Pak DEAE 8HR AP-1 Protein Mix
Adenosine
30 µL
A-9251
Carbonic Anhydras
120 µL
C-7500
Human Transferrine
300 µL
T-4515
Ovalbumin
240 µL
A-7641
Soybean Trypsin Inhibitor
300 µL
T-9003
Column: Protein-Pak SP 8HR AP-1 Protein Mix
Myoglobin
50 µL
M-0630
Ribonuclease
200 µL
R-5000
alpha-Chymotrypsinogen A
100 µL
C-4879
Cytochrome C
100 µL
C-7752
Lysozyme
100 µL
L-6876
Buffer A
200 µL