Waters AccQ-Tag Amino Acid Analysis Column - Manual

[ CARE AND USE MANUAL ] [ CARE AND USE MANUAL ]
I. INT RODUC T ION
This manual describes procedures for using the Waters AccQ•Tag ™
Amino Acid Analysis column for the separation of hydrolysate amino
acids derivatized according to the AccQ•Tag ™ Method.
The Waters AccQ•Tag ™ Amino Acid Analysis column is manufactured
in Waters.
ISO 9002-certified facilities under strict guidelines to assure the high-
est quality products. Please take a few moments to read this manual
carefully. Follow its recommendations to ensure reproducible chroma-
tography and to maximize column lifetime.
a. Waters AccQ•Tag ™ Column Description
The AccQ•Tag ™ column separates the hydrolysate amino acid deriva-
tives produced in the reaction with Waters AccQ•Fluor ™ reagent. The
AccQ•Tag ™ column is a high-efficiency 4 μm Nova-Pak ™ C
18
column
specifically certified for use with the AccQ•Tag ™ Method.
When you use the AccQ•Tag ™ column as part of a Waters AccQ•Tag ™
system, sub-picomole detection limits can be achieved.
b. Specifications
CONTENTS
I. INTRODUCTION
a. Waters AccQ•Tag ™ Column Description
b. Specifications
II. INSTALLING THE COLUMN
a. Preparing the System
b. Installing the Columns
III. USING THE COLUMN
a. Chromatography Guidelines
b. Efficiency Testing
c. Equilibrating the Column for the AccQ•Tag ™ Method
IV. CARE AND MAINTENANCE
a. Storing the Column
APPENDIX A. SPARE PARTS
APPENDIX B. WARRANTY/SERVICE INFORMATION
ACCQ•TAG ™ AMINO ACID ANALYSIS COLUMN
Column AccQ•Tag ™ Amino Acid Analysis Column
Dimensions 3.9 mm x 150 mm
Particle Size 4 μm
Packing Material Silica base bonded with C
18
Shipped in Acetonitrile/water (50%/50%)