Waters Ultrahydrogel Columns - Manual (Page 2)

[ Care and Use ManUal ]
Ultrahydrogel Columns 2
II. InstallatIon
a. Solvents
Ultrahydrogel columns are packed in distilled water for shipping.
Table 1 shows the maximum solvent replace¬ment flow rate for each
Ultrahydrogel column. Perform solvent changeover at a lower flow
rate than that shown in Table 2 for analysis.
CAUTION: ULTRAHYDROGEL COLUMNS ARE FLOW (PRESSURE) SENSI-
TIVE AND EXTREME CARE MUST BE TAKEN NOT TO EXCEED MAXIMUM
FLOW RATE ON START-UP.
b. Column
Table 1. Flow Rates for Replacing Solvents
NOTE: CHANGE SOLVENTS AS SELDOM AS POSSIBLE. FREQUENT
SOLVENT EXCHANGES ACCELERATE THE DEGRADATION OF COLUMN
EFFICIENCY.
c. Organic Solvents
Ultrahydrogel columns are physically and chemically stable in
ordinary water-soluble organic solvent mix¬tures. Aqueous solutions
of methanol, ethanol, acetonitrile, formic acid, and dimethylsulfoxide
also provide dependable separations. Organic solvent concentration
should not exceed 20%. It is possible however, to use organic sol-
vents up to 50% concentration by gradual introduction via gradient
method.
d. Aqueous Salt and Buffer Solution
Although some non-ionic samples can be analyzed in distilled water,
most water soluble polymers require aqueous salt or buffer solutions.
Some typical aqueous salt solutions:
• Sodium sulfate
• Sodium acetate
• Sodium dihydrogenphosphate
• Ammonium acetate
• Ammonium formate
Compatible buffer solutions:
• Phosphate
• Tris phosphate
• Tris hydrochloric acid
• Citrate
• Tris acetate
• Acetate
e. Flow Rate
For best resolution and maximum column life the flow rate should
not exceed the maximum flow rate listed below. Use Table 2 below to
determine recommended flow rates and maximum pressures.
Table 2. Recommended Flow Rates
Column Column Size Max. Flow Rate During
Solvent Change
Ultrahydrogel (all pore sizes) 7.8 mm i.d. x 30 cm 0.3 ml/min
Ultrahydrogel DP 7.8 mm i.d. x 30 cm 0.3 ml/min
Ultrahydrogel DNA 7.8 mm i.d. x 30 cm 0.3 ml/min
Ultrahydrogel Linear 7.8 mm i.d. x 30 cm 0.3 ml/min
Column Typical Operating Flow
Rate (mL/min)
Maximum Flow Rate
(mL/min)
Ultrahydrogel 120 0.5-0.8 1.0
Ultrahydrogel 250 0.5-0.8 1.0
Ultrahydrogel 500 0.3-0.6 1.0
Ultrahydrogel 1000 0.3-0.6 1.0
Ultrahydrogel 2000 0.3-0.6 1.0
Ultrahydrogel Linear 0.3-0.6 1.0
Ultrahydrogel DP 0.5-0.8 1.0