Waters HSPgel Columns User Manual
Care and use manual, Hspgel columns for high-speed gpc analysis
 
[ Care and Use ManUal ]
HSPgel Columns for High Speed GPC Analysis
1
Contents
I. IntroduCtIon
II. HsPgel Column FamIlIes
III. ConneCtIng tHe Column to tHe gPC system
a. Column Connectors 
b. Connecting an HSPgel Column 
c. Solvents
IV. Column temPerature ranges
I. IntroduCtIon
Thank you for choosing a Waters HSPgel
™
column. Every column
is individually tested and Certificates of Batch Analysis and a 
Performance Chromatogram are provided with each column. It is not 
recommended to use a guard column with the HSPgel columns, as it 
will add significant volume and possible additional bandspreading 
to the system. It is recommended, however, that a ~0.5 µm in-line 
filter (pre-column) be installed to trap any particulates that may 
potentially plug the column frit. The GPC analysis solvent must 
be filtered prior to use through an appropriate ~0.5 µm filter. The 
sample solutions should also be filtered prior to injection, unless the 
sample molecular weight is very high and there may be a chance of 
shear degradation.
HsPgel Columns For HIgH-sPeed gPC analysIs