Waters Carbamate Analysis Column - Manual (Page 5)

[ Care and Use ManUal ]
Carbamate Analysis Column 5
Table 1: Troubleshooting the Carbamate Analysis Column
b. Shutdown and Storage
Between Analyses
During the course of a working day, between analyses, continue
to pump the initial mobile phase mixture through the column. This
will maintain the equilibrium in the column necessary for good
retention time reproducibility. If a few hours will pass before the
next injection, the flow rate may be slowed down in the interim to a
few tenths of a mL/min to conserve solvent.
Overnight
When shutting down overnight or over a weekend, first flush the
column with 15-30 mL of 50/50 v/v acetonitrile/methanol. Then
turn the flow rate to zero mL/min and leave the column connected
in the system. The oven temperature may be maintained at 30 °C,
if desired.
Long-term Storage (more than 72 hours)
Flush the column with 15-30 mL of 50/ v/v acetonitrile/methanol.
Then turn the pump off. Allow the column to cool to ambient
temperature. Disconnect the inlet and outlet tubes from the column
and join them with a union. Install the end plugs in the column inlet
and outlet fittings. Tighten the end plugs firmly in place with 5/16”
open end wrench.
CAUTION: Do not overtighten – Overtightening will damage the
column fittings. Allowing columns to dry out may result in poor
chromatographic performance. Return the column to its box for
storage
VI. ColuMn and supplIes orderIng InforMatIon
Symptom Conditions Corrective Action
Excess pressure
buildup Filters plugged with
particulates. Replace filter element or clean
in an ultrasonic bath. Always
filter solvents and samples
Sample precipitates on
column (sample not
soluble in mobile
phase). Slowly purge with a solvent
appropriate to dissolve the
precipitate.
Clogged tubing. Replace tubing.
Loss of resolution,
broad peaks, or
low plate counts Mass overload Dilute sample and run it again.
Incorrect tubing size
(0.040” or 0.020”
i.d.) Install correct 0.009” ID stain-
less steel tubing to column
inlet & outlet.
Contaminated column. Slowly flush with a 50-100
mL of 50/50 v/v acetonitrile/
MeOH; then equilibrate with
initial mobile phase and run
sample again.
Insufficient equilibrium Continue equilibration
Filters partially plugged Replace or clean (inlet and
outlet) the Filter Retainer Disc
and the Filter.
Failing Injector Repair Injector.
Item Part Number
Carbamate Analysis Column, 3.9x150mm WAT035577
Filter Retainer WAT088084
Filter Retainer Disc WAT089567
In-Line Pre column Filter WAT084560
Solvent Clarification Kit with pump WAT085113
Solvent Clarification Kit without pump WAT085124
Aqueous Replacement Filters (HATF 04700),
pkg. of 100 WAT085147
Organic Replacement Filters (FHUP 04700),
pkg. of 100 WAT085118
Aqueous Sample Clarification Kit WAT026865
Guard-Pak Pre column Module: Kit WAT080040
Nova-Pak ® C
18
Guard-Pak Cartridges (10/Pkg.) WAT015220
Sep-Pak C
18
Cartridges (50/Box) WAT051910
Sep-Pak C
18
Plus Cartridges (24/Box) WAT011191
Sep-Pak C
18
Plus Cartridges (96/Box) WAT015402