Waters Glyco-Pak N Columns - Manual (Page 3)

[ Care and Use ManUal ]
Glyco-Pak N Columns 3
a. Mode of Action
The Glyco-Pak N column operates in a liquid/liquid partition mode. In this
mode, retention tunes and resolution are very sensitive to small changes in
the composition of the mobile phase. To insure retention time reproducibility,
mobile phase should be prepared by weight or in sufficiently large batches to
insure consistency.
Conditions
Sample:
Endo H released high mannose oligosaccharides
Mobile Phase: Acetonitrile/water (70:30)
Flow Rate: 1 ml/min
Detection: 197 nm
Figure 2: Endo H Released High Mannose Oligosaccharides
Conditions
Sample:
Mixed oligosaccharide standards
Mobile Phase: Acetonitrile/water (d8:32)
Flow rate: l ml/min
Detection: 200 nwm
Figure 3: Mixed Hydrazine Released Oligosaccharide Standards
(See Figure 4 for structures.)
Figure 4: Neutral Oligosaccharide Structures*
*Separation of Reducing Oligosaccharides Derived from Glycoproteins by HPLC
on a Polymeric Support, Bendiak, B.; On, J.; Brockhausen, I.; Vella, G.; Phoebe,
C.; in press.