Waters Analytical Columns and Cartridges - Manual (Page 5)

[ Care and Use ManUal ]
Analytical Columns and Cartridges 5
c. Storing the Column
For Column and Cartridge Columns: Leaving the column unused for
less than four days does not require special storage procedures.
For longer storage, store the column in shipping solvent which is
identical to the column test solvent with a few exceptions. Store
Nova-Pak CN HP and μBondapak CN columns in 0.1 M ammonium
sulfate. Store all μBondapak C18 columns in methanol and store
all μBondapak Phenyl columns in acetonitrile/water. If the mobile
phase contains a buffer salt, first flush the column with 10 column
volumes of 18 megohm water. Make sure that the end plugs are
firmly in place. Never let the column dry out.
For Radial-Pak Column Segments: Wash Radial-Pak column
segments with water to remove all buffer salts, then with the
organic component of the mobile phase. Use a miscible intermediate
if necessary. Remove the cartridges from the 8 x 10 holder and
replace the end caps.
V. t roubleshootIng
Changes in retention time, resolution or back pressure are often
due to column contamination. This is discussed in Section IV. b.,
Cleaning and Regenerating the Column.
Poor chromatography is often the result of a system problem. For
information on troubleshooting your system refer to the Guide to
Successful Operation of your LC System, available from Waters. Ask
for part number WAT022378.
Our experienced team of chromatographic specialists provide
outstanding customer support for corrective, preventive, and quality
maintenance. Our technical support staff is always available to help
you with questions and provide solutions to your applications needs.
In the U.S., call us at 1-800-252-4752. If you are outside the U.S.,
contact your local Waters representative.
VI. orderIng and addItIonal InformatIon
If you would like new product information or a copy of the Waters
Parts and Supplies Catalog, complete the reply card and mail. For
more detailed information about Waters columns and cartridges,
consult the Waters Chromatography Columns and Supplies Catalog
or contact your Waters representative. To obtain a copy of the
catalog, complete the enclosed reply card. The catalog is also
available on the Internet at www.waters.com.