Waters Atlantis T3, DC18 and HILIC Silica Columns User Manual
Care and use manual, Atlantis t3, dc, And hilic silica columns

[ Care and Use ManUal ]
Atlantis Columns
1
I. Int roductIon
Thank you for choosing an Atlantis
®
Column. The manufacture of Atlantis
columns begins with ultrapure reagents to control the chemical composition
and purity of the final product. Atlantis columns are manufactured in a cGMP,
ISO 9001:2000 certified plant with each step being conducted within nar-
row tolerances. Every column is individually tested and Certificates of Batch
Analysis and a Performance Chromatogram are provided with each column.
Waters recommends the use of Sentry
™
guard columns to maximize column
lifetime and protect the column from contaminants.
contents
I. IntroductIon
II. connectInG tHe coLuMn or cArtrIdGe to tHe
III. systeM ModIfIcAtIon recoMMendAtIons
HPLc systeM
IV. WAters sMALL PArtIcLe sIze (3 µM) coLuMns
– fAst cHroMAtoGrAPHy
V. coLuMn equILIbrAtIon
VI. coLuMn InstALLAtIon Procedure
VII. coLuMn PerforMAnce VALIdAtIon
VIII. InItIAL coLuMn effIcIency deterMInAtIon
IX. coLuMn usAGe
XII. HILIc GettInG stArted
XIII. coLuMn cLeAnInG, reGenerAtInG And storAGe
XIV. troubLesHootInG
AtLAntIs t3, dc
18
And HILIc sILIcA coLuMns