Bio-Rad Firmware & Software Updates - Manual (Page 136)

CFX96 Touch, CFX96 Touch Deep Well, CFX Connect, and CFX384 Touch Systems Instruction Manual
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2. Entire study: one inter-run calibrator is selected by the user and applied to the entire gene
study.
For more information, please contact Technical Support (phone numbers on back cover).
Gene Study Window
The Gene Study window includes two tabs:
• Study Setup tab. Click this tab to manage the runs in the Gene Study. Adding or
removing data files in a Gene Study does not change the original data in that file
• Study Analysis tab. Click this tab to view the gene expression data for the combined
runs
Figure 86 shows the Gene Study window, including the Study Setup and Study Analysis tabs.
Figure 86. Study Setup tab in the Gene Study window.
Study Setup Tab
Before importing data into a Gene Study, do the following in the Data Analysis window:
• Check that samples containing the same content are named with the same name. In
a Gene Study, the software assumes that wells with the same Target or Sample
name contain the same samples
• Adjust the baseline and threshold (C
q
) in the Quantification tab to optimize the data
in each run before you add them to a Gene Study
• Select the well group you want to include in the Gene Study
The Study Setup tab (Figure 86) shows a list of all the runs in the Gene Study.
• Add runs. Click the Add Data Files button to select a file from a browser window. To
quickly add runs to a Gene Study, drag the data files (.pcrd extension) to the Gene Study
window
TIP: In order to show data from one well group in the Gene Study, that group must
be selected before importing the data file.
• Remove runs from this Gene Study. Select one or more files in the list and click
Remove
• Add notes about the Gene Study. Type in the Notes box to add comments about the
files and analysis in this Gene Study