Bio-Rad Firmware & Software Updates - Manual (Page 152)

CFX96 Touch, CFX96 Touch Deep Well, CFX Connect, and CFX384 Touch Systems Instruction Manual
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For quantification data, select the following settings:
• Baseline Setting. Select the default baselining method for analysis mode. Choose
Baseline Subtracted Curve Fit, No Baseline Subtraction, or Baseline Subtracted
• Cq Determination Mode. Select between Regression mode or Single Threshold mode
to determine how C
q
values are calculated for each fluorescence trace
• Threshold Calc. Mode. Select between Auto Threshold and Manual Threshold mode. If
manual is chosen, the position of the threshold will be set to a specified relative
fluorescence unit (RFU) value. Data files from subsequent runs will use this threshold
setting
• Baseline Calc. Mode. Select between Auto and Manual Baseline mode. If manual is
chosen, the cycle range used to determine the baseline for all traces will be set based on
the values specified. Data files from subsequent runs will use this cycle range
• Log View. Select On to show a semilogarithmic graph of the amplification data. Select
Off to show a linear graph
For the end point data, select the following settings. Select the number of end cycles to
average when calculating the end point calculations:
• PCR. Enter a number of cycles for PCR to average the end cycles for quantification data
(default is 5)
• End Point Only Run. Enter a number of cycles for End Point Only Run to average the
end cycles for end point data (default is 2)
For the melt curve data, select to detect either positive or negative peaks.
For the well selector panes, select to label wells with sample type, target name, or sample
name.
Gene Expression Tab
Select the Gene Expression tab in the User Preferences window (Figure 96) to specify the
default settings for a new Gene Expression data file.
Figure 96. Gene Expression tab in the User Preferences window.