Bio-Rad Model 583 Gel Drying Supports - Manual (Page 10)

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Gel Preparation Techniques
3.0 GEL PREPARATION TECHNIQUES
Techniques for drying the following gels are discussed in this section:
• Sequencing Gels
• Acrylamide Protein Gels < 14%
• Gradient gels, gels >15%, and 1.5 mm thick gels
Warning
Use of enhancers such as PPO and DMSO will void the manufacturer’s
warranty.
Gels dried prior to autoradiography require a non-porous plastic film cover such as Saran Wrap ™ . The film,
which prevents the Sealing Gasket from becoming contaminated, can be peeled away and discarded as
radioactive waste.
To prevent the porous Gel Support from becoming contaminated with radioactivity, always use two sheets of
filter paper Use one sheet for the gel and the second sheet to absorb contamination from the gel.
For gels without radioactive label, a single sheet of filter paper for gel backing is all that is required.
Figure 3. Arrangement for Drying Gels
POROUS GEL SUPPORT
FILTER PAPER REQUIRED FOR
GELS WITH RADIOACTIVE LABEL
GEL'S FILTER PAPER BACKING
GEL
NON-POROUS PLASTIC SHEET
(SUCH AS SARAN WRAP) FOR
GELS WITH RADIOACTIVE LABEL
SEALING GASKET