Bio-Rad Model 583 Gel Drying Supports - Manual (Page 11)

8
Gel Preparation Techniques
Table 4
Drying Gels
Step Procedure
1. For gels with radioactive label,
place a dry sheet of filter paper on
top of the porous Gel Support.
2. Use a second sheet of filter paper
to mount the gel, and place the gel
in the gel dryer.
3. For gels with radioactive label,
cover the gel with a thin non-
porous plastic film, such as Saran
Wrap
™ .
4. Position the transparent Sealing
Gasket over the gel and close
the lid.
5. Turn on the vacuum pump.
Description
This procedure is necessary only for gels with radioac-
tive label.
a. Cut a second piece of the filter paper that is slightly
larger than the gel. This will serve as the gel back-
ing.
b. Place the gel on this second piece of filter paper.
If the gel has dried out, lightly mist the surface of
the gel with water prior to applying the filter paper.
c. Gently roll a pipette over the filter paper to remove
bubbles trapped between the gel and filter paper.
d. Place the gel and its filter paper backing in the gel
dryer.
Trim the plastic wrap to a size slightly larger than the
gel. The plastic wrap should not interfere with the
Sealing Gasket.
Make sure the gel, filter paper, and plastic wrap are
not interfering with the Sealing Gasket.
Verify the dryer has formed a vacuum seal. The
Sealing Gasket should appear slightly depressed in
the middle.