0 suggested drying times – Bio-Rad Model 583 Gel Drying Supports User Manual
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Suggested Drying Times
4.0 SUGGESTED DRYING TIMES
Gel drying time is a function of temperature, vacuum, gel porosity, and gel thickness. With the exception of
gels containing scintillant fluors, gels are not damaged by excessive drying.
The following table provides approximate drying times for various gel concentrations, given the dryer’s tem-
perature and vacuum are kept at 80° C and 28” Hg, respectively. Deviations from these guidelines are given
below and are based on the use of additional materials or other environmental settings:
•
At vacuum levels of 22” Hg to 25” Hg, add 50% to the drying times.
•
At 60°C, add 100% to the drying times.
•
Gels > 14% T may crack at a vacuum < 25” Hg.
Table 5
Suggested Drying Times for Single Percentage Gels
Acrylamide Concentration
Slab Thickness
Time at 80° C
3%
to
10%
0.375 mm
30 min.
10%
to
20%
0.375 mm
30 min.
3%
to
10%
0.5 mm
40 min.
10%
to
20%
0.5 mm
40 min.
3%
to
10%
0.75 mm
40 min. to 60 min.
10%
to
20%
0.75 mm
60 min. to 120 min.
3%
to
10%
1.5 mm
45 min. to 60 min.
10%
to
20%
1.5 mm
60 min. to 120 min.
3%
to
10%
3.0 mm
60 min. to 120 min.
10%
to
20%
3.0 mm
120 min. to 180 min.