Bio-Rad Model 583 Gel Drying Supports User Manual
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9
Gel Preparation Techniques
Table 4 (continued)
Drying Gels
Step
Procedure
6.
Program the drying cycle.
Description
For Sequencing gels:
a.
Select the
Sequencing
cycle.
b.
For normal rapid drying, the default temperature is
80°C.
For gels containing fluors for autoradiography, set
the temperature to 60°C.
c.
Select a drying time. Refer to Chapter 4, Drying
Times, for recommended drying times.
For gels having < 14% constant concentration acry-
lamide:
a.
Select the
PAGE
cycle.
b.
For normal rapid drying, set a temperature of
80°C.
For gels containing fluors for autoradiography,
select 60°C.
c.
Select a drying time. Refer to Chapter 4 for rec-
ommended drying times.
For gradient gels, gels having > 15% constant concen-
tration acrylamide, and 1.5 mm gels:
a.
Begin by soaking the gel for about one hour in a
500 ml solution of 3% glycerol (5%, if the gradient
is very steep), 40% MeOH, and 10% HOAc.
Gently agitate during the entire soak period.
b.
Select the
GRADIENT
cycle.
c.
Set a temperature of 60°C to 70°C.
d.
Select a drying time. Refer to Chapter 4 for rec-
ommended drying times.