Bio-Rad Sub-Cell® Model 192 Cell - Manual (Page 13)

Figure 2.1 Sealing the UVTP tray for gel casting.
7. Place the comb(s) into the appropriate slot(s) of the tray (refer to Section 2.2 for comb
adjustments).
8. When the solution of agarose has cooled to 60 °C (Section 2.1), pour the molten agarose
onto the tray.
Warning: Hot agarose (>60 °C) may cause the tray to warp or craze and will decrease the
lifetime of the tray. Warping may also result in sample wells of uneven depth.
9. Allow 30 – 60 minutes for the gel to solidify at room temperature.
10. Carefully remove the comb from the solidified gel.
11. Disengage the cam peg by turning and lifting upward. Slide the movable wall away from
the tray. Remove the tray from the gel caster.
Note: While the gel is solidifying, a light seal is formed between the gasket and the gel
(especially for low percentage agarose gels (<0.8%). Carefully lift the tray on one side to
release the seal.
12. Place the tray onto the leveled Sub-Cell base so that the sample wells are near the cathode
(black). DNA samples will migrate towards the anode (red) during electrophoresis.
13. Submerge the gel beneath 4 to 6 mm of electrophoresis buffer (Section 3.1).
10
Movable Well
Lift cam lever up
UVTP Gel Tray
Fixed wall
Leveling Feet
Gel Caster
Leveling Bubble
Engage and seal
(press down and rotate)
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