Bio-Rad Sub-Cell® Model 192 Cell - Manual (Page 19)

3.4 DNA and RNA Sample Loading Dye 1-2
A convenient 10x sample buffer stock consists of 50% glycerol, 0.25% bromophenol
blue, and 0.25% xylene cyanole FF in 1x electrophoresis buffer. Prepare only 1-10 ml of the
10x loading dye.
3.5 Gel Staining Solution
Add 10 mg of ethidium bromide to 1 ml distilled water. Bio-Rad offers pre-mixed
EtBr solutions (10 mg/ml). Store reagent in the dark.
Section 4
Care and Maintenance
4.1 Cleaning Sub-Cell System Components
1. All Sub-Cell systems parts should be washed with a mild detergent solution in warm
water. If necessary, use a soft-bristled brush or sponge to remove dried buffer salts or
agarose.
Note: Be careful not to snag or break the electrode wire in the base while cleaning.
2. Rinse all parts thoroughly with warm water or distilled water and air dry, if possible.
3. To clean recirculation ports and tubing, simply pump distilled water into the tubing to
rinse. Thoroughly empty tubing of liquid before use.
4.2 Compatible Cleaning Agents
Chemically compatible cleaners must be used to ensure long life of parts. These include:
• Aqueous solutions of soaps and mild detergents:
Bio-Rad Cleaning Concentrate (catalog number 161-0722)
Dishwashing Liquid
• Organic Solvents:
Hexane
Aliphatic Hydrocarbons
Do not leave plastic parts to soak in detergents more than 30 minutes. A short detergent
rinse typically is all that is required.
Caution: Do not use the following chemicals to clean Sub-Cell parts. Exposure to these
chemicals may cause the plastic parts to crack, craze, etch or warp.
• Chlorinated Hydrocarbons
Carbon Tetrachloride
Chloroform
• Aromatic Hydrocarbons
Benzene
Phenol
Toluene
Methyl Ethyl Ketone
Acetone
• Alcohols
Methanol
Ethanol
Isopropyl Alcohol
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