Bio-Rad Sub-Cell® Model 192 Cell - Manual (Page 14)

Tape Method
1. Seal the ends of the UVTP gel tray securely with strips of standard laboratory tape. Press
the tape firmly to the edges of the gel tray to form a fluid-tight seal.
2. Level the gel tray on a leveling table or workbench using the leveling bubble provided with
the instrument.
3. Place the comb(s) into the appropriate slot(s) of the tray (refer to Section 2.2 for comb
adjustments).
4. When the solution of agarose has cooled to 60 °C (Section 2.1), pour the molten agarose
onto the tray.
Warning: Hot agarose (>60 °C) may cause the tray to warp or craze and will decrease the
lifetime of the tray. Warping may also result in sample wells of uneven depth.
5. Allow the gel to solidify at room temperature for 30 – 60 minutes.
6. Carefully remove the comb from the solidified gel.
7. Remove the tape from the edges of the gel tray. Be careful when removing tape so the gel
does not slide off the tray.
8. Place the tray onto the leveled Sub-Cell base so that the sample wells are near the cathode
(black). DNA samples will migrate towards the anode (red) during electrophoresis.
9. Submerge the gel under 4 to 6 mm of electrophoresis buffer.
2.4 Recirculation Ports
Buffer recirculation is not required for most run conditions on the Sub-Cell systems. We
recommend buffer recirculation for extended run times (over 2 hours) or for high voltage
run conditions (150-200 volts). This will prevent lane distortion that can arise from uneven
heating or buffer pH gradients. If recirculation is desired, the buffer recirculation kit
(Bio-Rad catalog number 170-4537) contains the adapters required to connect the pump
tubing to the Sub-Cell lid.
1. Carefully remove the port plugs from the safety lid.
2. Turn clockwise and tighten the elbow-shaped recirculation port fitting into the threaded
port holes (Figure 2.2).
Note: There should be at least three threads extending below the bottom surface of the
safety lid.
Figure 2.2. Connecting the recirculation ports and tubing.
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Elbow-shaped
Fitting
Safety Lid
Recirculation Port
Straight Fitting
Tubing