Bio-Rad Sub-Cell® Model 192 Cell - Manual (Page 21)

4.5 RNase Decontamination
Sub-Cell parts can be cleaned with a mild detergent and treated for 10 minutes with 3%
hydrogen peroxide (H
2
O
2
) and then rinsed with 0.1% DEPC- (diethyl pyrocarbonate) treated
distilled water to eliminate RNases prior to using the Sub-Cell systems for RNA gels 1-2 . Do
not soak Sub-Cell parts in DEPC water. Consult references 1-2 for other suggestions regarding
the use of DEPC in RNase decontamination.
Caution: DEPC is a suspected carcinogen. Always wear gloves, eye glasses and a
laboratory coat. Use caution when handling DEPC-containing solutions. Consult the
DEPC MSDS for more information.
Do not attempt to RNase decontaminate Sub-Cell parts using extreme dry heat.
Note: Several commercial products are also available for eliminating RNase contamination.
RNaseZAP
™ (Ambion) or RNase AWAY ™ (Molecular Bio-Products) are safe, simple and
effective methods that if used properly do not craze or fog the Sub-Cell parts. See
manufacturer instructions for proper use.
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