Bio-Rad Sub-Cell® Model 192 Cell - Manual (Page 9)

Contruction
Base Cast Acrylic
Gel Casting Gates Anodized Aluminum
Safety Cover Cast Acrylic
Banana Plug/Electrode Cassette Polycarbonate
Banana Plugs Gold-Plated Brass, 4.4 cm Length
Electrodes Platinum, 0.25 mm Diameter
Electrical Cables Dual, 20 AWG, Tinned Copper Wire Cable
Flame-Retardant Polyurethane Insulation jacket
Electrical Leads Nickel Silver
Gel Tray UV-Transparent Acrylic Plastic (UVTP)
Combs Machined Acrylic
Comb Holder Polycarbonate
Gel Casting Device Polycarbonate
0.64 cm Silicon Foam
* Base buffer volumes will vary depending on the size and thickness of gel used.
Section 2
Operating Instructions
Note: Refer to Section 3 for information on preparation of RNA gels. See References
1 and 2 for more information on DNA and RNA electrophoresis.
2.1 DNA Gel Preparation
DNA agarose gels can be used to separate and visualize DNA of various sizes. Before
casting an agarose gel, consult Table 2.1 to determine the appropriate percent agarose gel to
use based on the size of DNA to be separated.
Procedure
1. Determine the amount of agarose (grams) and volume needed. Use Tables 2.1 and 2.2
as a guide for agarose concentration and gel volume requirements.
Example: For a 1% agarose gel, add 1 gram of agarose to 100 ml of electrophoresis buffer.
Table 2.1 Gel Concentration Required for DNA Separation 1-2
Gel Concentration % DNA Size (Kbp)
0.50 1 – 30
0.75 0.8 – 12
1.00 0.5 – 10
1.25 0.4 – 7
1.50 0.2 – 3
2-5* 0.01 – 0.5
* Sieving agarose such as Bio-Rad AmpliSize ® agarose.
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