Bio-Rad Bio-Plex Pro Human Acute Phase Reagent and Diluent Kits - Manual (Page 24)

20
1. Select Calibrate and confirm that the default values for CAL1
and CAL2 are the same as the values on the Bio-Plex calibration
bead labels. Use the Bio-Plex low RP1 target value.
2. Select OK and follow the instructions for CAL1 and CAL 2 calibration.
Prepare Protocol
Lot-specific assay panel protocols are available for download at
www.bio-rad.com/bio-plex/standards
If the desired protocol is not available for download, create a new protocol:
1. Open a new protocol by selecting File, then New from the main
menu. Locate the steps at the left of the protocol menu.
2. Select Step 1 (Describe Protocol) and enter information about the assay.
3. Select Step 2 (Select Analytes) and choose the appropriate acute phase
panel, from the assay pull-down menu. If the panel is not listed, create
a new panel and select the “Bio-Plex Pro” assay type from the assay
pull-down menu. See the Bio-Plex Manager software instruction
manual for details.
NOTE: It is important to select the appropriate assay type in the assay
pull-down menu to ensure that data is acquired using the correct bead
map and gate settings.
4. Choose the analytes to be selected for the assay from the available
list of targets. Note the targets will already be entered in the selected
view when using a downloaded protocol. In future runs, the selected
panel can be copied into the “available” view.
5. Select Step 3 (Format Plate) and click on the Plate Formatting tab.
Select the number of replicates, the autofill direction, and click on
the
icon. Drag the cursor over all the wells that contain standards.
Repeat by replacing the with , , and to identify all the
wells that contain blanks, controls, and samples, respectively.