Bio-Rad Bio-Plex Pro Human Acute Phase Reagent and Diluent Kits - Manual (Page 34)

Section 11
Troubleshooting Guide
This troubleshooting guide addresses problems that may be encountered with
Bio-Plex Pro™ acute phase assays. If you experience any of the problems listed
below, review the possible causes and solutions provided. This will assist you in
resolving problems directly related to how the assay steps should be performed.
Poor assay performance may also be due to the Bio-Plex array reader. To
eliminate this possibility, we highly recommend use of the Bio-Plex validation kit.
This kit will validate all the key functions of the array reader and assist the user in
determining whether or not the array reader is functioning properly.
Possible Causes Possible Solutions
30
High Inter-Assay %CV
Standards were not
reconstituted consistently Incubate the reconstituted
standards for 30 min on ice. Always
be consistent with the incubation
time and temperature.
Reconstituted standards and
diluted samples were not stored
properly Reconstituted standards and diluted
samples should be prepared on ice
as instructed. Prior to plating, the
reconstituted standards and diluted
samples should be equilibrated to
room temperature.
High Intra-Assay %CV
Bottom of filter plate not dry
Dry the bottom of the filter plate with
absorbent paper towel (preferably
lint-free) to prevent cross-
contamination.