Bio-Rad Bio-Plex Pro Human Acute Phase Reagent and Diluent Kits - Manual (Page 28)

Bio-Plex Manager Software Version 4.1 and 4.1.1
Prepare System
1. Empty the waste bottle and make sure that there is sufficient sheath
fluid in the bottle or cube before proceeding.
2. Turn on the reader, XY platform and HTF (if included). Allow the
system to warm up for 30 min.
3. Select Start up and follow the instructions to prepare the reader
to acquire data. If the system is idle for 4 hr without aquiring data, the
lasers will automatically turn off. To reset the 4-hour countdown, select
Warm up and wait for the optics to reach operational temperature.
Calibrate With Low RP1 Target Value
Calibrate using Bio-Plex calibration beads and the
low RP1 target on the
CAL2 bottle. This software version requires the user to calibrate either on
the low or high RP1 target value. Daily calibration is recommended before
acquiring data.
1. Select Calibrate and confirm that the default values for CAL1 and
CAL2 are the same as the values on the Bio-Plex calibration bead
labels. Use the Bio-Plex low RP1 target value.
2. Select OK and follow the instructions for CAL1 and CAL2 calibration.
Prepare Protocol
1. Open a new protocol by selecting File, then New from the main
menu. Locate the steps at the left of the protocol menu.
NOTE: To minimize protocol setup, previously prepared lot-specific
Bio-Plex Pro acute phase assay protocols in BPM 4.0 are available
for download at www.biorad.com/bio-plex/standards. The user
will need to select the 25 region bead map in Step 7 (Run Protocol).
2. Select Step 1 (Describe Protocol) and enter information about the
assay.
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