Bio-Rad Bio-Plex Pro Human Acute Phase Reagent and Diluent Kits User Manual
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NOTE: If the preset protocol was downloaded from the web site, the
standards will already be added to the plate format. Make any
necessary changes to the plate layout to match your plate requirements.
6. Select Step 4 (Enter Standards Info) to enter standards information.
Note that this information will already be entered when using a
download protocol. Otherwise:
a)
Deselect the box labeled “Same concentration values for all
analytes”. If analyzing a 5-plex assay, also deselect the box labeled
“Same units for all analytes”.
b)
Select the first analyte from the pull-down cell.
c)
Click on the Enter Automatically button and then select the
highest concentration value (typically S1).
d)
Enter the value for the highest concentration. The information is
included on the product insert provided with the assay.
e)
Enter the dilution factor (typically 4) and select Calculate. The
concentrations for each replicate point of the standard will be
populated for the selected analyte.
f)
Repeat steps 6b through 6e for each analyte in the assay.
Plate Formatting Example
21