Bio-Rad Bio-Plex Pro Human Acute Phase Reagent and Diluent Kits User Manual
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5. Select Step 4 (Enter Standards Info) to enter standards information.
Note that this information will already be entered with the preset
download protocol.
a)
Select each analyte individually from the pull-down cell. Deselect
the box labeled “Same concentration values for all analytes”. If
analyzing a 5-plex assay, also deselect the box labeled “Same
units for all analytes”.
b)
Select the Enter Automatically option and then select the most
concentrated value as S1.
c)
Enter the concentration of S1 from the product insert provided
with the assay.
d)
Enter the dilution factor as 4 and select Calculate. The
standards information will be populated fro the selected analyte.
e)
Repeat steps 5a through 5d for each analyte in the assay.
6. Select Step 5 (Enter Controls Info) to enter controls information. This
is where the concentration of the user-specified controls is entered
into the protocol.
a)
Select each analyte individually from the pull-down cell. Deselect
the box for same concentration values for all analytes.
b)
Enter the description and dilution information for each user-
specified control.
c)
Repeat steps 6a and 6b for each analyte in the assay.
7. Select Step 6 (Enter Sample Info) and enter sample information.
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