Bio-Rad Bio-Plex Pro Human Acute Phase Reagent and Diluent Kits User Manual
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7. Select Step 5 (Enter Controls Info) to enter controls information. This
is where the concentration of the user-specified controls is entered
into the protocol.
a)
If necessary, deselect the box for same concentration values for
all analytes.
b)
Select an analyte from the pull-down cell.
c)
Enter the description and dilution information for each user-
specified control.
d)
Repeat steps 7b and 7c for each additional analyte in the assay.
8. Select Step 6 (Enter Sample Info) and enter sample information.
Remember to enter the appropriate dilution factor information.
9. Select Step 7 (Run Protocol). Then:
a)
Choose
100 beads per region.
b)
In Advanced Settings, set the bead map to
25 region.
c)
In Advanced Settings, set the sample size to
50 µl.
d)
In Advanced Settings, confirm that the DD gate is set to
5,000
(low) and
32,000 (high).
e)
Save the protocol (.pbx) file.