Bio-Rad Model EM-1 Econo™ UV Monitor - Manual (Page 14)

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When the light stops flashing and remains lit, the Model EM-1
Econo UV Monitor is ready for use. Wait at least 20 minutes if an
AUFS setting of 0.05 or less is to be used.
Note: If the Model EM-1 Econo UV Monitor is used in a cold room
or cold box, leave the lamp on continuously to prevent the formation
of condensation.
3. Start the flow of buffer or solvent, and continue for several minutes
prior to starting a separation or measurement.
4. Select an AUFS range using the arrow selector keys on the front
panel of the control unit.
5. Press the Auto-Zero key on the front panel until the yellow light at
the bottom of the graphic display starts flashing. When it stops
flashing, adjust the chart recorder baseline.
Section 7
Care and Maintenance
The Model EM-1 Econo UV Monitor requires very little mainte-
nance to assure reliable operation. Following are general procedures for
maintenance of the UV monitor and optics module.
Model EM-1 Econo UV Monitor control unit and optics module
case: During normal operation, spills and splashes may cause residues to
form on the component cases. Use a squirt bottle and soapy water to
wash down the outer cases of the instruments. The instruments are
resistant to spills. However, unplug first, and take care to prevent water
from entering the vents.
Optics module: Clean the outside surfaces of the optics module as
described above. The 280 nm and 254 nm filters in the optics unit should
be cleaned only when necessary with a dry lens tissue and replaced in
the optics module. Use of a dilute ethanol or isopropanol solution will
assist in the removal of contaminants. When the optics module is not in
use, disconnect and clean out dissolved salts and protein from the flow
cell using a syringe filled with distilled water. Store the flow cell by
injecting a dilute solution (10-25%) of ethanol or isopropanol into the
cell to prevent microbial growth. Use the end caps provided to seal the
flow cell’s inlet and outlet lines. To prevent the formation of salt
crystals and solute deposits, do not allow the flow cell to dry out.
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