Bio-Rad Bio-Scale™ Mini UNOsphere SUPrA™ Affinity Cartridges - Manual (Page 19)

Section 4
Getting Started
4.1 Screening Buffers and Conditions for
UNOsphere SUPrA™
Buffers
A1: 0.02 M sodium phosphate, 0.02 M sodium citrate,
pH 7.5.
A2: 0.02 M boric acid, 0.02 M sodium phosphate,
0.02 M sodium citrate, 1.0 M sodium sulfate, pH 9.0.
B: 0.02 M sodium citrate, 0.1 M sodium chloride,
pH 2.5.
Conditions
Equilibrate column: with 10 CV buffer A1 or A2 (see
note below).
Inject: MAb sample either as is or diluted 1:10 into
buffer A1 or A2 (see below).
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