Bio-Rad Bio-Scale™ Mini UNOsphere SUPrA™ Affinity Cartridges - Manual (Page 22)

Bio-Scale™ Mini cartridges are available in 1 and 5 ml
cartridge format. The UNOsphere SUPrA media are
also available in larger amounts from 25 ml bottles
to bulk quantities.
Section 5
Care of the Cartridge
5.1 Cleaning
During operation it is recommended that the column
bed is periodically cleaned to remove bound
substances that can adversely impact the separation
performance of the column. The accumulated
substances fall into two general categories: a)
difficult to remove precipitated or denatured substances,
and b) substances that are hydrophobically bound
to the column bed. To ensure that all bound substances
are released and washed out of the column, the
following cleaning-in-place (CIP) protocols are
recommended.
17
10014997A:10005454A.qxd 9/23/2008 3:16 PM Page 17