Bio-Rad Bio-Scale™ Mini UNOsphere SUPrA™ Affinity Cartridges User Manual
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Wash: buffer A1 or A2 until effluent absorbance
returns to baseline.
Elute: in a 10 CV linear gradient to 100% B, or
Desired % buffer B.
Strip: with 5 CV buffer B.
Use buffer A1 for binding human and guinea pig
IgG. Use buffer A2 for all others. The recommended
column equilibration interval is excessive under
most conditions, but should be used as a default
until specific equilibration requirements are established
for your particular system. In addition, be aware of
solubility limitations of antibodies that require high
salt concentrations (A2 buffer) for binding.
Characterize product solubility thoroughly under
loading conditions. If the antibody fails to remain
fully soluble for the longest possible duration from
equilibration to completion of sample load when
adjusted to load conditions, then load by using
online dilution technique.
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