Bio-Rad Bio-Scale™ Mini UNOsphere SUPrA™ Affinity Cartridges - Manual (Page 23)

CIP Protocols
The following protocols are suggested to remove
precipitated or denatured substances from the bed.
Wash the bed with 2–5 column volumes in reverse
flow with one of the following solutions:
• 6 M guanidine hydrochloride
• 10 mM hydrochloric acid
• 0.1 M sodium hydroxide
• 1 M acetic acid/20% ethanol
Followed by a reverse flow wash with at least
5 column volumes of binding buffer, neutral pH (7–8).
To remove any hydrophobically bound substances
from the bed, wash the column with 2–5 column
volumes in reverse flow of a nonionic
surfactant/detergent, followed by a reverse-flow wash
with at least 5 column volumes of neutral pH binding
buffer.
18
10014997A:10005454A.qxd 9/23/2008 3:16 PM Page 18