Bio-Rad Affi-Gel Blue Gel - Manual (Page 10)

Table 2. Affi-Gel Blue Gel Enzyme Applications
Enzyme Source Eluant Reference
1-aminocyclopropane- Tomato pericarp tissue 50 mM KPO
4
, 41
1-carboxylate synthase 5 µM pyridoxal phosphate
3',5' cyclic AMP Dictyostelium 0.18 M NaCl 3
phosphodiesterase discoideum
3'-PGA diesterase Yeast 0.6 M KCl in 50 mM Tris-HCl 35
5-methyl-L-tetrahydrofolate Bovine liver 0.4-3.0 M KCl 6
reductase
Acylation stimulating protein
Human plasma 3 M NaCl, 0.02 M phosphate 37
Adenylate cyclase Bovine brain 1 mM KCl, 8 mM ATP, 24
16 mM MgCl
2
, 1 mM EDTA
Alkaline phosphatase E. coli - 39
Alkyl hydroperoxide reductase S. thphimurium 1.0 M KCl, 0.5 M NaCl 36
ATP:AMP phosphotransferase Bovine heart 0.4-2.0 M NaCl 10
Calmodulin-dependent cyclic Bovine brain 0.15-1.5 M NaCl 23
nucleotide phosphodiesterase
Carbamyl phosphate synthetase
Frog liver 1 mM dithiothreitol 14
DNA polymerase Calf thymus 0-0.5 M KCl 12
Formamidopyrimidine-DNA E. coli 0.1-0.8 M KCl 4
glycosylase
Glutamate dehydrohenase
Yeast 10 mM NADH, 1 M NH4Cl 15
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