Bio-Rad Affi-Gel Blue Gel - Manual (Page 3)

Section 1
Introduction
Affi-Gel blue affinity gel is a beaded, crosslinked
agarose gel with covalently attached Cibacron ® Blue
F3GA dye. It contains
≥
1.9 mg dye per ml of gel, and
has a capacity for albumin binding of greater than 11
mg/ml. Affi-Gel blue gel purifies a large range of
proteins from widely divergent origins. The blue dye
functions as an ionic, hydrophobic, aromatic, or
sterically active binding site in various applications.
Proteins that interact with Affi-Gel blue gel can be
bound or released with a high degree of specificity by
manipulating the composition of the eluant buffers. In
many cases, one can also predict what will interact with
the matrix and the general conditions under which
binding and elution will occur.
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