Bio-Rad Affi-Gel Blue Gel - Manual (Page 6)

Section 3
Instructions for Use
3.1 Sample Preparation
Proper adjustment of the sample pH and ionic
strength is critical for consistent and reproducible results
when using dye affinity gels. The sample must be
exchanged into the appropriate application buffer. This
can be achieved by exchanging it into the application
buffer using Econo-Pac 10DG desalting columns,
Bio-Gel P-6DG desalting gel, or the Econo-Pac P6
cartridge. The choice of product depends on the sample
volume. Alternatively, the sample can be dialyzed
against the application buffer. All samples should be
filtered through a 0.45 µm filter.
3.2 Albumin Removal
Affi-Gel blue gel provides a simple first step in the
purification of many serum proteins by removing the
major serum constituent, albumin. The binding of albumin
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